Coding

Part:BBa_K3819128:Design

Designed by: Yujiao Peng   Group: iGEM21_UESTC-China   (2021-09-25)


Aspergillus niger lipase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After extensive screening, we finally selected lipase from Aspergillus Niger（DQ647700.1） and optimized the codon suitable for heterologous expression of Pichia pastoris. Depending on the vectors and promoters we use, we remove the incompatible sites, especially those at both ends, so that the enzyme and other components can be assembled normally.


Source

Gene ID：DQ647700.1，https://www.ncbi.nlm.nih.gov/nuccore/DQ647700.1

References

1.Shu Z, Duan M, Yang J, Xu L, Yan Y. Aspergillus niger lipase: Heterologous expression in Pichia pastoris, molecular modeling prediction and the importance of the hinge domains at both sides of the lid domain to interfacial activation. Biotechnol Prog. 2009 Mar-Apr;25(2):409-16. doi: 10.1002/btpr.147. PMID: 19248178.